Glycogen-bound polyphosphate kinase from the archaebacterium Sulfolobus acidocaldarius.

نویسندگان

  • R Skórko
  • J Osipiuk
  • K O Stetter
چکیده

Glycogen-bound polyphosphate kinase has been isolated from a crude extract of Sulfolobus acidocaldarius by isopycnic centrifugation in CsCl. Divalent cations (Mn2+ greater than Mg2+) stimulated the reaction. The enzyme does not require the presence of histones for its activity; it is inhibited strongly by phosphate and slightly by fluoride. The protein from the glycogen complex migrated in a sodium dodecyl sulfate-polyacrylamide gel as a 57-kilodalton protein band; after isoelectric focusing it separated into several spots in the pH range of 5.6 to 6.7.

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عنوان ژورنال:
  • Journal of bacteriology

دوره 171 9  شماره 

صفحات  -

تاریخ انتشار 1989